Translational_Unit
LucTS

Part:BBa_K5490023:Design

Designed by: IOANNIS VASILEIOS ELAFROPOULOS   Group: iGEM24_IOANNINA   (2024-09-25)


LUCIFERASE WITH TARGET SITES IN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1693
    Illegal XbaI site found at 1915
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1693
    Illegal NotI site found at 1898
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1693
    Illegal BamHI site found at 1906
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1693
    Illegal XbaI site found at 1915
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1693
    Illegal XbaI site found at 1915
    Illegal NgoMIV site found at 74
    Illegal NgoMIV site found at 1451
    Illegal NgoMIV site found at 1472
    Illegal NgoMIV site found at 1787
    Illegal AgeI site found at 1166
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NONE


Source

Photinus pyralis

References